Enterococci are important human pathogens in both community an_d hospital infection an_d exhibit intrinsic resistance to various antibiotics. In recent years, enterococcal infections have become major therapeutic challenge because of their increased incidence an_d spread of strains that have acquired resistance to several antimicrobial agents. However, little is known aboat species distribution an_d epidemiology of antibiotic resistance among Enterococci isolated from environmental sources. One source of resistance Enterococci may be coastal waters. This study was performed during summer an_d autumn season. out of 60 samples which collected in ten prefectures in western regions of Guilan province ( port of Anzali) were analysed for presence of resistant Enterococci to the common antibiotics. we also have measured parameters including temperature of waters, turbidity, dissolved oxygen, pH an_d salinity. Enumeration of bacteria performed by MPN method. After confirmation of isolated Enterococcus spp. by biochemical an_d differential tests, antibiotic susceptibility pattern were performed by agar disk diffusion method. Determination of MIC for heavy metals from among of isolated Enterococci microdilution broth technique. Out of 60 samples, 46 specimens were positive. The results showed that with decreasing of temperature during autumn an_d winter season, the number of Enterococci in different places were reduced. The isolated strans of Enterococci were E. faecium, E. faecalis, E. casseliflavus, E. gallinarum an_d E. raffinosus. The results indicated that all of isolated Enterococci, were resistant to vancomycin, penicillin, gentamicin an_d erythromycin. Identification of Enterococcus spp. All of Enterococci strans showed high-level resistance against copper, zinc an_d ferrous. in recreational can determine their ecological an_d epidemiological characterisitics, so the prevalence of resistant Enterococci, treatment of the infectious diseases would be more problematic an_d costly.
Key words: Enterococcus spp., coastal waters, MPN method, antibiotic susceptibility pattern.